Effects of macromolecules recovered from uterine luminal fluid on the metabolism of [u-14c]glucose by mouse morulae and early blastocysts in vitro

Khurana, N.K. and Wales, R.G. (1989) Effects of macromolecules recovered from uterine luminal fluid on the metabolism of [u-14c]glucose by mouse morulae and early blastocysts in vitro. Reproduction, Fertility and Development, 1 (2). pp. 89-98.

Abstract

Day-4 mouse embyros grew well in culture media supplemented with macromolecular components of uterine fluids recovered on day 3, 4 or 5 of pregnancy and pseudopregnancy. Addition of these components to media during a 2-h pulse culture had no significant effect on the incorporation of glucose carbon by morulae/early blastocysts. However, various fractions of uterine luminal macro­molecules significantly increased the turnover of glucose carbon incorporated into acid-soluble and acid-insoluble glycogen, into nucleic acids and into proteins during a 24-h chase culture. These effects were due mainly to components with a molecular weight between 1000 and 10 000 Da and the activity was most marked in fluids collected on day 5 of pregnancy or pseudopregnancy. Oxidation of glucose during a 4-h incubation was inhibited in the presence of certain uterine macro­molecules but most consistently by the large molecular weight component (>300 000 Da). Some differences were noted in the inhibitory activity of macromolecules obtained from pregnant and pseudopregnant sources. There was little evidence of an effect of uterine-fluid components on lactate production from glucose.

Item Type:	Journal Article
Murdoch Affiliation(s):	School of Veterinary Studies
Publisher:	CSIRO Publishing
Copyright:	© CSIRO 1989.
URI:	http://researchrepository.murdoch.edu.au/id/eprint/34248

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