Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Effects of macromolecules recovered from uterine luminal fluid on the metabolism of [u-14c]glucose by mouse morulae and early blastocysts in vitro

Khurana, N.K. and Wales, R.G. (1989) Effects of macromolecules recovered from uterine luminal fluid on the metabolism of [u-14c]glucose by mouse morulae and early blastocysts in vitro. Reproduction, Fertility and Development, 1 (2). pp. 89-98.

Link to Published Version: http://dx.doi.org/10.1071/RD9890089
*Subscription may be required

Abstract

Day-4 mouse embyros grew well in culture media supplemented with macromolecular components of uterine fluids recovered on day 3, 4 or 5 of pregnancy and pseudopregnancy. Addition of these components to media during a 2-h pulse culture had no significant effect on the incorporation of glucose carbon by morulae/early blastocysts. However, various fractions of uterine luminal macro­molecules significantly increased the turnover of glucose carbon incorporated into acid-soluble and acid-insoluble glycogen, into nucleic acids and into proteins during a 24-h chase culture. These effects were due mainly to components with a molecular weight between 1000 and 10 000 Da and the activity was most marked in fluids collected on day 5 of pregnancy or pseudopregnancy. Oxidation of glucose during a 4-h incubation was inhibited in the presence of certain uterine macro­molecules but most consistently by the large molecular weight component (>300 000 Da). Some differences were noted in the inhibitory activity of macromolecules obtained from pregnant and pseudopregnant sources. There was little evidence of an effect of uterine-fluid components on lactate production from glucose.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary Studies
Publisher: CSIRO Publishing
Copyright: © CSIRO 1989.
URI: http://researchrepository.murdoch.edu.au/id/eprint/34248
Item Control Page Item Control Page