Effects of oxygen concentration on the metabolism of [u-14c]glucose by mouse morulae and early blastocysts in vitro

Khurana, N.K. and Wales, R.G. (1989) Effects of oxygen concentration on the metabolism of [u-14c]glucose by mouse morulae and early blastocysts in vitro. Reproduction, Fertility and Development, 1 (2). pp. 99-106.

Abstract

The utilization of the acid-soluble glycogen pool in pulse-labelled embryos was significantly enhanced during 24- and 48-h chase culture under low oxygen concentrations of 5, 2-5 and 1%. The lower the oxygen tension the greater was the turnover in the pool. The morphological development of embryos was equally as good at very low oxygen concentrations as when embryos were cultured in 5-20% oxygen. Reduction in oxygen concentration enhanced the oxidative utilization of substrate, as measured by rate of carbon dioxide production. The present study could provide an explanation for the discrepancy in glycogen content between mouse blastocysts developing in utero and in vitro and for the reported beneficial effects of low oxygen concentration during development of embryos in culture.

Item Type:	Journal Article
Murdoch Affiliation(s):	School of Veterinary Studies
Publisher:	CSIRO Publishing
Copyright:	© CSIRO 1989.
URI:	http://researchrepository.murdoch.edu.au/id/eprint/34247

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