Effects of coculture with uterine epithelial cells on the metabolism of glucose by mouse morulae and early b1astocysts

Khurana, N.K. and Wales, R.G. (1987) Effects of coculture with uterine epithelial cells on the metabolism of glucose by mouse morulae and early b1astocysts. Australian Journal of Biological Sciences, 40 (4). pp. 389-395.

Abstract

Coculture of mouse morulae/early blastocysts with isolated endometrial epithelial cells reduced incorporation of glucose carbon into embryonic glycogen but had no significant effect on incorporation into other internal carbon pools during a 5-h culture in serum-supplemented Dulbecco’s modification of Eagle’s minimum essential medium. Turnover of glycogen pools during 24-h chase culture of pulse-labelled embryos was unaffected by the presence of uterine epithelial cells recovered from day-4 pregnant or non-pregnant mice. However, significantly more label was retained in non-glycogen macromolecules during chase in the presence of endometrium recovered from non-pregnant than from pregnant uteri.

Item Type:	Journal Article
Murdoch Affiliation(s):	School of Veterinary Studies
Publisher:	CSIRO Publishing
Copyright:	© 1987 ASEG.
URI:	http://researchrepository.murdoch.edu.au/id/eprint/34246

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