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Agonist-induced vasoactive responses in isolated perfused porcine dental pulpal arterioles

Yu, C.Y., Boyd, N.M., Cringle, S.J., Su, E.N., Alder, V.A. and Yu, D.Y. (2002) Agonist-induced vasoactive responses in isolated perfused porcine dental pulpal arterioles. Archives of Oral Biology, 47 (2). pp. 99-107.

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A novel isolated perfused pulpal arteriole preparation and microperfusion system was used to evaluate the direct vasoactive responses of pulpal arterioles to selected agonists. Short lengths of porcine pulpal arterioles (101.7 ± 2.2 μm o.d., n = 105) were dissected out and placed in an environment-controlled bath on the stage of an inverted microscope. Both ends of the vessel were cannulated and perfused at a controlled rate through the lumen. The diameter of the vessel was measured online. Following equilibration, the vessel was challenged with various agonists: adrenaline (epinephrine), noradrenaline (norepinephrine), phenylephrine, dopamine, isoproterenol, 5-hydroxytryptamine, histamine and adenosine. The endothelium-dependent vasodilator acetylcholine was used to evaluate endothelial cell function. Adrenaline, noradrenaline, phenylephrine, 5-hydroxytryptamine and dopamine caused dose-dependent contractions (adrenaline = noradrenaline > phenylephrine > dopamine > 5-hydroxytryptamine). Isoproterenol and histamine provoked a dose-dependent dilation. Adenosine produced pronounced vasodilatation in vessels precontracted with 10-8 M endothelin-1. Functional adrenergic, histamine, 5-hydroxytryptamine and adenosine receptors are, therefore, present in porcine pulpal arterioles. The isolated perfused pulpal arteriole preparation may prove valuable in understanding local control mechanisms of pulpal microcirculation.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Journal or Publication Title: Archives of Oral Biology
Page Range: pp. 99-107
Publisher: Elsevier
Copyright: © 2002 Elsevier Science Ltd.
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