Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Properties of organic acid utilization mutants of Rhizobium leguminosarum strain 300

Arwas, R., McKay, I.A., Rowney, F.R.P., Dilworth, M.J. and Glenn, A.R. (1985) Properties of organic acid utilization mutants of Rhizobium leguminosarum strain 300. Microbiology, 131 (8). pp. 2059-2066.

Free to read:
*No subscription required


Summary: Mutants of Rhizobium leguminosarum 300 which were unable to utilize one or more organic acids as growth substrates were obtained by Tn5 mutagenesis. Mutant strain MNF3080 was defective in dicarboxylate transport and was unable to grow on succinate. Strain MNF3085 was defective in phosphoenolpyruvate carboxykinase and hence could not carry out gluconeogenesis. This strain did not grow on pyruvate, succinate, glutamate or arabinose but grew on glucose and on glycerol. Strain MNF3075 was unable to utilize pyruvate; the biochemical lesion in this mutant was not identified. MNF3085 and MNF3075 were symbiotically effective. MNF3080 nodulated peas, but the nodules were ineffective in N2 fixation and displayed morphological abnormalities. These data support previous findings which suggest that utilization of exogenous dicarboxylates is essential for effective nodule development by R. leguminosarum.

Item Type: Journal Article
Murdoch Affiliation(s): School of Environmental and Life Sciences
Publisher: Society for General Microbiology
Item Control Page Item Control Page