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Effects of prostaglandins E-2 and F-2 on the metabolism of [U-14C]glucose by mouse morulae-early blastocysts in vitro

Khurana, N.K. and Wales, R.G. (1987) Effects of prostaglandins E-2 and F-2 on the metabolism of [U-14C]glucose by mouse morulae-early blastocysts in vitro. Reproduction, 79 (1). pp. 275-280.

Free to read: http://dx.doi.org/10.1530/jrf.0.0790275
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Abstract

Summary. During 5-h culture in the presence of radioactive glucose, PGE-2 (10 μg/ml) significantly inhibited incorporation of glucose into the acid-soluble glycogen pool. PGE-2 at 1 and 10 μg/ml and PGF-2α at 1 μg but not 10 μg/ml stimulated incorporation of glucose into non-glycogen macromolecules during culture. However, the utilization of acid-soluble glycogen and other biochemical pools was not affected by the presence of PGs in the medium during 24-h chase culture of pulse-labelled embryos. Carbon dioxide production was significantly suppressed in the presence of PGs but accumulation of lactate was not affected. The results indicate that PGE-2 and PGF-2α, in physiological concentrations, directly influence the metabolism of glucose by preimplantation embryos.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary Studies
Publisher: Society for Reproduction and Fertility
URI: http://researchrepository.murdoch.edu.au/id/eprint/34021
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