Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay

Chan, D., Barratt, J., Roberts, T., Phillips, O., Šlapeta, J., Ryan, U.ORCID: 0000-0003-2710-9324, Marriott, D., Harkness, J., Ellis, J. and Stark, D. (2016) Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay. Veterinary Parasitology, 227 . pp. 42-47.

PDF - Authors' Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (665kB) | Preview
Link to Published Version:
*Subscription may be required


Dientamoeba fragilis is a potentially pathogenic, enteric, protozoan parasite with a worldwide distribution. While clinical case reports and prevalence studies appear regularly in the scientific literature, little attention has been paid to this parasite’s biology, life cycle, host range, and possible transmission routes. Overall, these aspects of Dientamoeba biology remain poorly understood at best. In this study, a total of 420 animal samples, collected from Australia, were surveyed for the presence of Dientamoeba fragilis using PCR. Several PCR assays were evaluated for sensitivity and specificity. Two previously published PCR methods demonstrated cross reactivity with other trichomonads commonly found in animal samples. Only one assay exhibited excellent specificity. Using this assay D. fragilis was detected from one dog and one cat sample. This is the first report of D. fragilis from these animals and highlights the role companion animals may play in D. fragilis transmission. This study demonstrated that some published D. fragilis molecular assays cross react with other closely related trichomonads and consequently are not suitable for animal prevalence studies.

Item Type: Journal Article
Murdoch Affiliation(s): School of Veterinary and Life Sciences
Publisher: Elsevier BV
Copyright: © 2016 Elsevier B.V.
Item Control Page Item Control Page


Downloads per month over past year