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In vitro germination of wheat pollen on raffinose medium

Cheng, C. and McComb, J.A. (1992) In vitro germination of wheat pollen on raffinose medium. New Phytologist, 120 (4). pp. 459-462.

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Wheat pollen gave up to 81.7 % germination when cultured on a 0.7 % agar medium containing 100 mg 1−1 H3BO3, 300 mg 1−1 CaCl2.2H2O and 0.75 M raffinose. On this medium at 25°C, germination started within 5 min and tubes reached a mean maximum length of 200 μm after 1 h. Maltose supported 27.9% germination, while sucrose gave at most 6.8%. Pollen on media with fructose, galactose, dextrose or lactose failed to germinate.

Item Type: Journal Article
Murdoch Affiliation(s): School of Biological and Environmental Sciences
Publisher: Blackwell Publishing
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