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Rapid detection of H and R Panton–Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Tong, S.Y.C., Lilliebridge, R.A., Holt, D.C., Coombs, G.W.ORCID: 0000-0003-1635-6506, Currie, B.J. and Giffard, P.M. (2010) Rapid detection of H and R Panton–Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis. Diagnostic Microbiology and Infectious Disease, 67 (4). pp. 399-401.

Link to Published Version: http://dx.doi.org/10.1016/j.diagmicrobio.2010.03.0...
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Abstract

We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC).

Item Type: Journal Article
Publisher: Elsevier Inc.
Copyright: © 2010 Published by Elsevier Inc.
URI: http://researchrepository.murdoch.edu.au/id/eprint/30774
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