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Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

Abraham, S., Chin, J., Brouwers, H.J.M., Turner, B., Zhang, R. and Chapman, T.A. (2011) Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins. Applied and Environmental Microbiology, 77 (18). pp. 6691-6693.

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Abstract

Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

Item Type: Journal Article
Publisher: American Society for Microbiology
Copyright: © 2011, American Society for Microbiology.
URI: http://researchrepository.murdoch.edu.au/id/eprint/30393
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