Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particles
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Orbán, L., Hahn, E. and Chrambach, A. (1988) Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particles. Electrophoresis, 9 (4). pp. 167-171.
Link to Published Version: http://dx.doi.org/10.1002/elps.1150090404
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Abstract
Discontinuous buffer systems operative between pH 5.7 and 7.4, 0 °C, were generated, which are characterized by more rapidly displaced moving boundaries than applied previously. These allow one to resolve subcellular particles relatively rapidly and at relatively low agarose gel concentrations. A commercial mixture of DNA restriction fragments pre-stained with ethidium bromide was found to be a suitable tracking dye for these boundaries.
| Publication Type: | Journal Article |
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| Publisher: | John Wiley & Sons Ltd |
| Copyright: | © 1988 VCH Verlagsgesellschaft mbH |
| URI: | http://researchrepository.murdoch.edu.au/id/eprint/30350 |
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