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Physical identification of a virus in a crude leaf extract by its ferguson plot in agarose gel electrophoresis

Orbán, L. and Chrambach, A. (1988) Physical identification of a virus in a crude leaf extract by its ferguson plot in agarose gel electrophoresis. Electrophoresis, 9 (4). pp. 162-166.

Link to Published Version: http://dx.doi.org/10.1002/elps.1150090403
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Abstract

Crude extracts of turnip crinkle virus upon agarose gel electrophoresis yield (i) virus patterns unperturbed by contaminants; (ii) plots of mobility vs. gel concentration (Ferguson plots) parallel with those of the purified virus. The parallelism suggests similarity in size and shape but a lower net charge for the crude virus. This result is obtained when gel electrophoresis is carried out either in a continuous buffer or in a discontinuous (moving boundary electrophoresis) buffer system. The latter mode has the substantial benefit of electrophoretic (auto-)concentration of dilute virus sample prior to resolution. Thus, the Ferguson plot analysis in a discontinuous buffer system of turnip crinkle virus can be viewed as a model procedure for the physical identification of other viruses contained in dilute extracts, feasible even in the absence of a prior knowledge as to the nature of, or isolation of, the virus.

Item Type: Journal Article
Publisher: John Wiley & Sons Ltd
Copyright: © 1988 VCH Verlagsgesellschaft mbH
URI: http://researchrepository.murdoch.edu.au/id/eprint/30349
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