Detection of turnip crinkle virus on agarose gel electropherograms at the nanogram load level
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Orbán, L. and Chrambach, A. (1988) Detection of turnip crinkle virus on agarose gel electropherograms at the nanogram load level. Electrophoresis, 9 (7). pp. 299-302.
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Abstract
The previous conditions for the physical characterization of turnip crinkle virus (TCV) by quantitative agarose gel electrophoresis [1, 2] were limiting the method to the microgram load level and were therefore insufficiently sensitive to satisfy the need in many areas of virology for detection of viruses containing single-stranded RNA at the nanogram level. The present report remedies that defect by presenting a technique compatible with the nanogram load level of such viruses. The technique is based on a reduction of gel thickness and on the use of silver staining.
| Item Type: | Journal Article |
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| Publisher: | John Wiley & Sons Ltd |
| Copyright: | © 1988 VCH Verlagsgesellschaft mbH |
| URI: | http://researchrepository.murdoch.edu.au/id/eprint/30347 |
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