Novel polymorphic microsatellites from Guppy (Poecilia reticulata) and their utility in swordtails (Xiphophorus helleri)

Yue, G-H and Orbán, L. (2010) Novel polymorphic microsatellites from Guppy (Poecilia reticulata) and their utility in swordtails (Xiphophorus helleri). Current Research Journal of Biological Sciences, 2 (3). pp. 161-163.

Abstract

Ten microsatellites were isolated from a genomic DNA library generated from guppy (Poecilia reticulata; Poecilidae) enriched for CA-repeats. All of the 10 microsatellites were polymorphic in guppy with an average allele number of 4.9/locus ranging from 2 to 14. All 10-primer pairs amplified specific products in green swordtail (Xiphophorus helleri) and 9 of the 10 microsatellites displayed polymorphism (average allele number: 4.1/locus with a scope between 2 and 8). Size range of alleles at most loci were similar between the two fish species. These microsatellites could be applied to breeding programs performed on these two species and possibly other poecilids and to genetic and ecological studies.

Item Type:	Journal Article
Publisher:	Maxwell Science Publication
Copyright:	© 2010 Maxwell Scientific Organization
URI:	http://researchrepository.murdoch.edu.au/id/eprint/30238

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