Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs
Davis, J.ORCID: 0000-0002-7078-1645, Raisis, A.L., Cianciolo, R.E., Miller, D.W.
ORCID: 0000-0002-4634-5819, Shiel, R.E., Nabity, M.B. and Hosgood, G.L.
(2016)
Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs.
Veterinary Anaesthesia and Analgesia, 43
(3).
pp. 262-270.
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Abstract
Objective: To determine changes in urine neutrophil gelatinase-associated lipocalin concentration (uNGAL) in anaesthetized Greyhound dogs that developed acute tubular damage following haemorrhage and resuscitation with colloid-based fluids. Study design: Prospective experimental study. Animals: Seven healthy adult entire male Greyhound dogs. Methods: During isoflurane anaesthesia, approximately 50 mL kg-1 of blood was removed to maintain mean arterial pressure (MAP) ≤40 mmHg for 1 hour followed by gelatin-based colloid administration to maintain MAP ≥60 mmHg for 3 hours. Data, including oxygen extraction ratio and uNGAL, were collected before (T0) and immediately following (T1) haemorrhage, and hourly during reperfusion (T2-T4). After T4, dogs were euthanized and renal tissue was collected for histology. Statistical analysis was performed using repeated-measures one-way anova. Data are presented as means (95% confidence interval). Results: Histology identified renal tubular epithelial damage in all dogs. Urine NGAL concentration increased from 12.1 (0-30.6) ng mL-1 at T0 to 122.0 (64.1-180.0) ng mL-1 by T3. Compared with T0, uNGAL was significantly higher at T3 (p = 0.016) and was increased 24-fold. Conclusions and clinical relevance: Despite wide individual variation in baseline uNGAL, increases in uNGAL were observed in all dogs, suggesting that this biomarker has the potential to detect renal tubular injury following haemorrhage-induced hypotension and colloid-mediated reperfusion.
Item Type: | Journal Article |
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Murdoch Affiliation(s): | School of Veterinary and Life Sciences |
Publisher: | Blackwell Publishing Inc. |
Copyright: | © 2015 Association of Veterinary Anaesthetists and the American College of Veterinary Anesthesia and Analgesia |
URI: | http://researchrepository.murdoch.edu.au/id/eprint/29499 |
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