Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Molecular Biology: RNA polymerase II is required for RNAi-dependent heterochromatin assembly

Kato, H., Goto, D.B., Martienssen, R.A., Urano, T., Furukawa, K. and Murakami, Y. (2005) Molecular Biology: RNA polymerase II is required for RNAi-dependent heterochromatin assembly. Science, 309 (5733). pp. 467-469.

Link to Published Version: http://dx.doi.org/10.1126/science.1114955
*Subscription may be required

Abstract

In Schizosaccharomyces pombe, the RNA interference (RNAi) machinery converts pericentromeric transcripts into small interfering RNAs (siRNAs) and is required for the assembly of pericentromeric heterochromatin. Here we describe a mutation in the second largest subunit of RNA polymerase II (RNAPII). Both wild-type and mutant RNAPII localized to the pericentromere. However, the mutation resulted in the loss of heterochromatic histone modifications and in the accumulation of pericentromeric transcripts, accompanied by the loss of siRNAs. This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly.

Item Type: Journal Article
Publisher: American Association for the Advancement of Science
URI: http://researchrepository.murdoch.edu.au/id/eprint/28437
Item Control Page Item Control Page