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Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons

Ma, B., Savas, J.N., Yu, M-S, Culver, B.P., Chao, M.V. and Tanese, N. (2011) Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons. Scientific Reports, 1 . Article 140.

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Transport of mRNAs to diverse neuronal locations via RNA granules serves an important function in regulating protein synthesis within restricted sub-cellular domains. We recently detected the Huntington's disease protein huntingtin (Htt) in dendritic RNA granules; however, the functional significance of this localization is not known. Here we report that Htt and the huntingtin-associated protein 1 (HAP1) are co-localized with the microtubule motor proteins, the KIF5A kinesin and dynein, during dendritic transport of β-actin mRNA. Live cell imaging demonstrated that β-actin mRNA is associated with Htt, HAP1, and dynein intermediate chain in cultured neurons. Reduction in the levels of Htt, HAP1, KIF5A, and dynein heavy chain by lentiviral-based shRNAs resulted in a reduction in the transport of β-actin mRNA. These findings support a role for Htt in participating in the mRNA transport machinery that also contains HAP1, KIF5A, and dynein.

Item Type: Journal Article
Publisher: Nature Publishing Group
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