Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum

Tan, K.C., Trengove, R.D., Maker, G.L.ORCID: 0000-0003-1666-9377, Oliver, R.P. and Solomon, P.S. (2009) Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum. Metabolomics, 5 (3). pp. 330-335.

Abstract

A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916–1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease.

Item Type:	Journal Article
Murdoch Affiliation(s):	School of Pharmacy
Publisher:	Springer
Copyright:	Springer
URI:	http://researchrepository.murdoch.edu.au/id/eprint/2799

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