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Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides

Højland, T., Veedu, R.N., Vester, B. and Wengel, J. (2012) Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides. Artificial DNA: PNA & XNA, 3 (1). pp. 14-21.

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Abstract

We describe the first enzymatic incorporation of an α-L-LNA nucleotide into an oligonucleotide. It was found that the 5′-triphosphate of α-L-LNA is a substrate for the DNA polymerases KOD, 9°Nm, Phusion and HIV RT. Three dispersed α-L-LNA thymine nucleotides can be incorporated into DNA strands by all four polymerases, but they were unable to perform consecutive incorporations of α-L-LNA nucleotides. In addition it was found that primer extension can be achieved using templates containing one α-L-LNA nucleotide.

Item Type: Journal Article
Publisher: Taylor & Francis
Copyright: © 2012 Landes Bioscience
URI: http://researchrepository.murdoch.edu.au/id/eprint/27428
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