Cleavage and protection of locked nucleic acid–modified DNA by restriction endonucleases
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Crouzier, L., Dubois, C., Wengel, J. and Veedu, R.N. (2012) Cleavage and protection of locked nucleic acid–modified DNA by restriction endonucleases. Bioorganic & Medicinal Chemistry Letters, 22 (14). pp. 4836-4838.
Link to Published Version: http://dx.doi.org/10.1016/j.bmcl.2012.05.113
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Abstract
Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far. We herein for the first time report cleavage by restriction endonuclease of LNA-modified DNA oligonucleotides. The experiments revealed that RsaI is an efficient enzyme capable of recognizing and cleaving LNA-modified DNA oligonucleotides. Furthermore, introduction of LNA nucleotides protects against cleavage by the restriction endonucleases PvuII, PstI, SacI, KpnI and EcoRI.
| Item Type: | Journal Article |
|---|---|
| Publisher: | Elsevier BV |
| Copyright: | © 2012 Elsevier Ltd. |
| URI: | http://researchrepository.murdoch.edu.au/id/eprint/27413 |
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