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Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids

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Goubet, A., Chardon, A., Kumar, P., Sharma, P.K. and Veedu, R.N. (2013) Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids. Bioorganic & Medicinal Chemistry Letters, 23 (3). pp. 761-763.

Link to Published Version: http://dx.doi.org/10.1016/j.bmcl.2012.11.096
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Abstract

C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields. Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR.

Item Type: Journal Article
Publisher: Elsevier BV
Copyright: © 2012 Elsevier Ltd.
URI: http://researchrepository.murdoch.edu.au/id/eprint/27407
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