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In Vitro Incorporation of LNA Nucleotides

Veedu, R.N., Vester, B. and Wengel, J. (2007) In Vitro Incorporation of LNA Nucleotides. Nucleosides, Nucleotides and Nucleic Acids, 26 (8-9). pp. 1207-1210.

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An LNA modified nucleoside triphosphate 1 was synthesized in order to investigate its potential to act as substrate for DNA strand synthesis by polymerases. Primer extension assays for the incorporation experiments revealed that Phusion High Fidelity DNA polymerase is an efficient enzyme for incorporation of the LNA nucleotide and for extending strand to full length. It was also observed that pfu DNA polymerase could incorporate the LNA nucleotide but it failed to extend the strand to a full length product.

Publication Type: Journal Article
Publisher: Taylor and Francis
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