Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Diverse novel mesorhizobia nodulate New Zealand native Sophora species

Tan, H.W., Heenan, P.B., De Meyer, S.E., Willems, A. and Andrews, M. (2015) Diverse novel mesorhizobia nodulate New Zealand native Sophora species. Systematic and Applied Microbiology, 38 (2). pp. 91-98.

PDF - Authors' Version
Download (552kB)
Link to Published Version:
*Subscription may be required


Forty eight rhizobial isolates from New Zealand (NZ) native Sophora spp. growing in natural ecosystems were characterised. Thirty eight isolates across five groups showed greatest similarity to Mesorhizobium ciceri LMG 14989T with respect to their 16S rRNA and concatenated recA, glnll and rpoB sequences. Seven isolates had a 16S rRNA sequence identical to M. amorphae ATCC 19665T but showed greatest similarity to M. septentrionale LMG 23930T on their concatenated recA, glnll and rpoB sequences. All isolates grouped closely together for their nifH, nodA and nodC sequences, clearly separate from all other rhizobia in the GenBank database. None of the type strains closest to the Sophora isolates based on 16S rRNA sequence similarity nodulated Sophora microphylla but they all nodulated their original host. Twenty one Sophora isolates selected from the different 16S rRNA groupings produced N2-fixing nodules on three Sophora spp. but none nodulated any host of the type strains for the related species. DNA hybridisations indicated that these isolates belong to novel Mesorhizobium spp. that nodulate NZ native Sophora species.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Rhizobium Studies
Publisher: Elsevier GmbH.
Copyright: © 2014 Elsevier GmbH.
Item Control Page Item Control Page


Downloads per month over past year