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Transformation of Petunia hybrida by the Agrobacterium suspension drop method

Wylie, S.J., Tjokrokusumo, D. and McComb, J.A. (2003) Transformation of Petunia hybrida by the Agrobacterium suspension drop method. In: Jackson, J.F. and Linskens, H.F., (eds.) Genetic Transformation of Plants. Springer, Berlin, pp. 45-51.

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Plant transformation is a key methodology that has allowed transfer and expression of novel genes for the improvement of economically important plant species as well as enquiry into deeper questions about the function of plant genes. For many plant species, stable transformation remains difficult or impossible. Where it is possible, there is usually a need for expensive resources such as laminar flow hoods, controlled environment growth rooms and highly skilled practitioners. In addition, there are often problems related to combining efficient plant regeneration with gene transfer as transfer techniques are carried out in undifferentiated cell cultures. Low transformation efficiency, instability of transgene expression, somaclonal variation and inability to regenerate whole plants are common problems.

Item Type: Book Chapter
Murdoch Affiliation: School of Biological Sciences and Biotechnology
Publisher: Springer
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