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The Effect of hydroxyethyl starch 130/0.4 on canine platelet function

McBride, Duana (2014) The Effect of hydroxyethyl starch 130/0.4 on canine platelet function. Masters by Research thesis, Murdoch University.

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Hydroxyethyl starch (HES) 130/0.4 is an artificial colloid solution commonly used to treat shock in dogs. Adverse effects from this solution have been reported in people, including platelet dysfunction. It was unknown if HES 130/0.4 causes platelet dysfunction in dogs. Therefore, we investigated the in vitro and in vivo effects of HES 130/0.4 on platelet function in dogs. We used closure time (CT) using the platelet function analyser-100 and adenosine diphosphate-collagen cartridges as a platelet agonist, to measure platelet function in this report. In the in vitro study, two solutions were compared: HES 130/0.4 and HES 200/0.5. Citrated blood from ten healthy dogs was diluted 1:9 and 1:3 with HES 130/0.4, HES 200/0.5 and 0.9% sodium chloride (NaCl). Closure time of the diluted blood was measured. Only HES 200/0.5 increased the CT beyond the dilutional effect at the 1:3 dilution, to a median CT of 125 seconds (interquartile range, 117.5 to 139.5 seconds), suggesting that HES 200/0.5, but not HES 130/0.4, caused platelet dysfunction at this dilution. No effect of HES or dilution on CT was identified at the 1:9 dilution. In the in vivo study, haemorrhagic shock was induced by removing 48ml/kg of blood from eleven greyhounds under general anaesthesia. Dogs were randomised to receive either 20mL/kg of HES 130/0.4 or 80mL/kg of 0.9% NaCl intravenously over 20 minutes. Both the HES 130/0.4 and 0.9% NaCl group had a significantly increased mean CT after fluid administration to 91.4 seconds (95% CI 69.3-113.4) and 95.5 seconds (95% CI 78.2-112.8), respectively. The magnitude of change was significantly greater for the 0.9% NaCl group than the HES 130/0.4 group, indicating the increase in CT with HES 130/0.4 is most likely due to a dilutional effect. In this study we also investigated the effect of shock on platelet function by measuring CT before and after inducing haemorrhagic shock. We found haemorrhagic shock did not significantly change CT. This report suggests HES 130/0.4 does not cause significant platelet dysfunction in dogs.

Item Type: Thesis (Masters by Research)
Murdoch Affiliation(s): School of Veterinary and Life Sciences
Supervisor(s): Smart, Lisa and Hosgood, Giselle
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