Isolation and characterization of microsatellites in the bird-pollinated, autohexaploid, Eremophila glabrassp.glabra (R.Br. (Ostenf.)) (Myoporaceae), an Australian endemic plant

Elliott, C.P. (2009) Isolation and characterization of microsatellites in the bird-pollinated, autohexaploid, Eremophila glabrassp.glabra (R.Br. (Ostenf.)) (Myoporaceae), an Australian endemic plant. Molecular Ecology Resources, 9 (4). pp. 1242-1246.

Abstract

Thirty-eight microsatellite loci were developed for the bird pollinated, autohexaploid, Eremophila glabra ssp. glabra. A genomic library was screened with dinucleotide and trinucleotide sequence repeats. Polymorphism ranged from one to 21 alleles per locus. Twenty-four loci exhibited null alleles, based on patterns of inheritance between maternal and progeny phenotypes. Cross-species amplification of nine Eremophila species was successful for most primers, indicating wide applicability across the genus. These microsatellites will be used to study the gene flow patterns of fragmented populations of E. glabra ssp. glabra.

Item Type:	Journal Article
Publisher:	Wiley-Blackwell
Copyright:	© 2009 Blackwell Publishing Ltd
URI:	http://researchrepository.murdoch.edu.au/id/eprint/22531

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