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PCR for identification of spirochaetes associated with intestinal spirochaetosis

Park, N.Y., Chung, C.Y., McLaren, A.J., Atyeo, R.F. and Hampson, D.J.ORCID: 0000-0002-7729-0427 (1994) PCR for identification of spirochaetes associated with intestinal spirochaetosis. In: Proceedings of the 13th International Pig Veterinary Society Congress, 26 - 30 June, Bangkok, Thailand p. 197.

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Abstract

Weakly haemolytic intestinal spirochaetes, distinct from both Serpulina hyodysenteriae and Serpulina innocens, have been reported to cause a disease called intestinal spirochaetosis, or spirochaetal diarrhoea, in weaned pigs (1, 2, 3, 4) . Infected animals develop a sloppy mucoid diarrhoea and a reduction in growth rate. These spirochaetes are slightly more slender than S. innocens, and characteristically only have 4-6 axial flagellae ( 5). Strain P43/6/78, which was isolated from the first recorded case of porcine intestinal spirochaetosis ( 1), has been deposited with the American Type Culture Collection (as ATCC 51139) as the type strain of a possible new genus and species of spirochaete ( "Anguillina coli") ( 5). In the current study we have used a polymerase chain reaction (PCR) amplification of a section of the region encoding the 16s ribosomal RNA gene as a rapid and specific means of identifying these bacteria.

Item Type: Conference Paper
Murdoch Affiliation: School of Veterinary Studies
URI: http://researchrepository.murdoch.edu.au/id/eprint/22009
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