Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus

Boulos, S. and Rood, J.I. (1986) Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus. FEMS Microbiology Letters, 33 (1). pp. 73-78.

Link to Published Version: http://dx.doi.org/10.1016/0378-1097(86)90189-8
*Subscription may be required

Abstract

The fimbrial subunit gene from the benign type BBacteroides nodosus isolate AC/6 was cloned into theSphI site of the multicopy vector plasmid pUC19. FiveEscherichia coli recombinants that were positive in a colony immunoassay were shown, by Western transfer analysis, to produce an immunologically cross-reacting protein of identical molecular size to fimbrial subunits prepared fromB. nodosus AC/6. Restriction endonuclease analysis showed that 4 of the recombinant plasmids carried a 6.7 kbSphI fragment. Recloning experiments showed that the fimbrial subunit gene was located within a 2.5 kbEcoRI-SphI fragment and that there was aPstI site located within the structural gene or its regulatory region. These recombinant clones will prove useful for the construction of a multivalent recombinant vaccine for the control of ovine footrot.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary Studies
Publisher: Blackwell Publishing
URI: http://researchrepository.murdoch.edu.au/id/eprint/20685
Item Control Page Item Control Page