Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus

Boulos, S. and Rood, J.I. (1986) Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus. FEMS Microbiology Letters, 33 (1). pp. 73-78.

Abstract

The fimbrial subunit gene from the benign type BBacteroides nodosus isolate AC/6 was cloned into theSphI site of the multicopy vector plasmid pUC19. FiveEscherichia coli recombinants that were positive in a colony immunoassay were shown, by Western transfer analysis, to produce an immunologically cross-reacting protein of identical molecular size to fimbrial subunits prepared fromB. nodosus AC/6. Restriction endonuclease analysis showed that 4 of the recombinant plasmids carried a 6.7 kbSphI fragment. Recloning experiments showed that the fimbrial subunit gene was located within a 2.5 kbEcoRI-SphI fragment and that there was aPstI site located within the structural gene or its regulatory region. These recombinant clones will prove useful for the construction of a multivalent recombinant vaccine for the control of ovine footrot.

Item Type:	Journal Article
Murdoch Affiliation(s):	School of Veterinary Studies
Publisher:	Blackwell Publishing
URI:	http://researchrepository.murdoch.edu.au/id/eprint/20685

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