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A helicase gene (helO) in Rhizobium meliloti WSM419

Reeve, W.G., Tiwari, R.P.ORCID: 0000-0003-3354-770X, Dilworth, M.J. and Glenn, A.R. (1997) A helicase gene (helO) in Rhizobium meliloti WSM419. FEMS Microbiology Letters, 153 (1). pp. 43-49.

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A 2.8 kb BamHI DNA fragment adjacent to a BamHI fragment containing actR-actS (a sensor/regulator pair required for low pH tolerance in Rhizobium meliloti WSM419) was cloned and sequenced. A computer predicted protein of 821 amino acids, designated HelO, showed extensive similarity with 'DEAH' motif helicases. Expression of helO was higher at pH 7.0 than pH 5.8 and it did not require the product of the actR gene. Inactivation of helO by insertion of a Ω interposon at codon 40 did not affect nodulation, growth or tolerance to low pH, high temperature, osmolarity or elevated levels of copper or zinc.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Rhizobium Studies
Publisher: Blackwell Publishing
Copyright: (c) Blackwell Publishing
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