Carbon catabolism in continuous cultures and bacteroids of Rhizobium leguminosarum MNF 3841

McKay, I.A., Dilworth, M.J. and Glenn, A.R. (1989) Carbon catabolism in continuous cultures and bacteroids of Rhizobium leguminosarum MNF 3841. Archives of Microbiology, 152 (6). pp. 606-610.

Abstract

Bacteroids of R. leguminosarum MNF3841 isolated from pea nodules using Percoll gradients had activities of TCA cycle enzymes up to 6-fold higher than those measured in free-living cells grown on fumarate or sucrose. Activities of sugar catabolic enzymes on the other hand were 2-14-fold lower in isolated bacteroids than in sucrose-grown free-living cells. In continuous culture, cells of strain MNF3841 grown on sucrose under Pi limitation had 2-3-fold higher activities of invertase, glucose-6-phosphate dehydrogenase, the Entner-Doudoroff enzymes and 6-phosphogluconate dehydrogenase, than cells grown on fumarate. With one exception O2 limited cultures had similar activities of the carbon catabolic enzymes to Pi-limited cultures grown in the same substrate. Glucose-6-phosphate dehydrogenase in O2-limited cells grown of fumarate was 50% lower than in Pi-limited cells. Co-utilization of fumarate and sucrose occurred with chemostat cultures supplied with both under a variety of conditions.

Item Type:	Journal Article
Murdoch Affiliation(s):	School of Biological and Environmental Sciences
Publisher:	Springer Verlag
Copyright:	© 1989 Springer-Verlag
URI:	http://researchrepository.murdoch.edu.au/id/eprint/20409

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