Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi

Dobrowolski, M.P. and O'Brien, P.A. (1993) Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi. FEMS Microbiology Letters, 113 (1). pp. 43-47.

Link to Published Version: http://dx.doi.org/10.1016/0378-1097(93)90096-K
*Subscription may be required

Abstract

The products of RAPD-PCR amplification of Phytophthora cinnamomi DNA were separated by electrophoresis in agarose. Parallel Southern blots of the gels were hybridized with nick translated DNA from different species of Phytophthora. Fragments that hybridized specifically to P. cinnamomi DNA were identified. These fragments were purified and cloned into pUC18. Their specificity for P. cinnamomi was confirmed.

Item Type: Journal Article
Murdoch Affiliation(s): School of Biological and Environmental Sciences
Publisher: Blackwell Publishing
URI: http://researchrepository.murdoch.edu.au/id/eprint/19603
Item Control Page Item Control Page