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Temperature and other environmental effects on ammocoete organs in culture

Stephenson, E.M. and Potter, I.C. (1967) Temperature and other environmental effects on ammocoete organs in culture. Journal of Embryology and Experimental Morphology, 17 (3). pp. 441-452.

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The optimal temperature and the range suitable for culturing amphibian tadpole hearts in vitro have been established (Stephenson, 1967). In order that comparison with the larval stage of a phylogenetically distinct poikilothermic vertebrate might be made, whole hearts and chopped explants of other organs of ammocoete larvae of Mordacia mordax (Richardson) were cultured at temperatures ranging from 37 to 5%C. As one of us (I.C.P) is also carrying out field investigations relating to Mordacia, we hoped to discover whether the temperature range suitable for cultures in vitro could be correlated with the range occurring in the animal's natural environment. We also hoped to add to the very sparse information available regarding media requirements of cyclostome tissues in culture.

In Australia,Mordacia mordax has a range which extends from Tasmania in the south to a latitude of approximately 34°S.on the east coast (Strahan, 1960)

Item Type: Journal Article
Publisher: Company of Biologists Ltd
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