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Evaluation of novel platforms to differentiate pathovars of plant pathogenic bacteria

Hailstones, D.L., Lock, J.E., Berryman, D., Rochfort, S.J., Flack, M.L., Englezou, A., Jones, M.ORCID: 0000-0001-5002-0227, Cakir, M., Smuts, C. and Chapman, T.A. (2011) Evaluation of novel platforms to differentiate pathovars of plant pathogenic bacteria. In: Inaugural joint 4th Asian Conference on Plant Pathology and the 18th Biennial Australasian Plant Pathology Society Conference (2011), 26 - 29 April 2011, Darwin Convention Centre, Darwin, NT.

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Many of the biggest threats to the biosecurity of Australia’s plant industries are bacterial, but difficulties in identification to pathovar level could seriously delay incursion management and affect market access. Pathovars are defined by host specificity so bioassays remain the definitive means of identification, but require physical containment and can be slow and subjective. Some pathovar‐specific serological and molecular tests are available but better diagnostic methods are often required.

We have evaluated the use of proteomics and metabolomics, platforms that identify functional molecules potentially associated with plant‐pathogen interactions, to identify biomarkers that differentiate pathovars in Xanthomonas species.

The proteomics component has focused on profiling membrane‐associated proteins extracted from selected bacterial isolates. Profiles show isolates of the same pathovar cluster together and proteins are differentially expressed between distinct pathovars. Differentially expressed proteins have been analysed by digestions and mass spectrometry and the genes that encode them identified by reference to genomic sequences. Based on this information, molecular tests to differentiate the pathovars are being designed and validated.

The metabolomics component has analysed metabolite expression in selected bacterial pathovars. Results show separation between the different pathovars and differentially expressed metabolites are evident.

Item Type: Conference Item
Murdoch Affiliation(s): Western Australian State Agricultural Biotechnology Centre
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