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Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

Laczka, O., Skillman, L., Ditcham, W.G.F., Hamdorf, B., Wong, D.K.Y., Bergquist, P. and Sunna, A. (2013) Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts. Journal of Microbiological Methods, 95 (2). pp. 182-185.

Link to Published Version: http://dx.doi.org/10.1016/j.mimet.2013.08.014
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Abstract

We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5×102 Cryptosporidium oocysts per mL in 60min.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Life Sciences
Publisher: Elsevier BV
Copyright: © 2013 Elsevier B.V.
URI: http://researchrepository.murdoch.edu.au/id/eprint/18327
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