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Transgenic yellow lupin (Lupinus luteus)

Li, H., Wylie, S.J.ORCID: 0000-0002-5639-7460 and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (2000) Transgenic yellow lupin (Lupinus luteus). Plant Cell Reports, 19 (6). pp. 634-637.

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Transgenic yellow lupin (Lupinus luteus L.) plants have been generated by meristem co-cultivation with Agrobacterium tumefaciens. The binary plasmid pPZBNIa contains the bar gene under the control of a CaMV 35 S promoter. The transformation method involves inoculation of embryonic axis explants with A. tumefaciens, flooding the meristem with glufosinate, and initial culture on non-selective medium. Shoots were transferred to culture medium containing 20 mg/l glufosinate. Following subculture, shoots were grafted onto non-transgenic narrow-leafed lupin (L. angustifolius L.) seedling rootstocks, or rooted in vitro. The overall transformation efficiency, as determined at the T1 generation, was 0.05%–0.75%. The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses.

Item Type: Journal Article
Murdoch Affiliation(s): Western Australian State Agricultural Biotechnology Centre
School of Biological Sciences and Biotechnology
Publisher: Springer Verlag
Copyright: © Springer-Verlag Berlin Heidelberg 2000
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