Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

The biochemical toxicology of 1,3-difluoro-2-propanol, the major ingredient of the pesticide Gliftor: The potential of 4-methylpyrazole as an antidote

Feldwick, M.G., Noakes, P.S., Prause, U., Mead, R.J. and Kostyniak, P.J. (1998) The biochemical toxicology of 1,3-difluoro-2-propanol, the major ingredient of the pesticide Gliftor: The potential of 4-methylpyrazole as an antidote. Journal of Biochemical and Molecular Toxicology, 12 (1). pp. 41-52.

Link to Published Version: http://dx.doi.org/10.1002/(SICI)1099-0461(1998)12:...
*Subscription may be required

Abstract

Administration to rats of 1,3-difluoro-2-propanol (100 mg kg−1 body weight), the major ingredient of the pesticide gliftor, resulted in accumulation of citrate in the kidney after a 3 hour lag phase. 1,3-Difluro-2-propanol was found to be metabolized to 1,3-difluoroacetone and ultimately to the aconitate hydratase inhibitor (-) erythrofluorocitrate and free fluoride. The conversion of 1,3-difluoro-2-propanol to 1,3-difluoroacetone was found to be catalyzed by an NAD+-dependent alcohol dehydrogenase, while the defluorination was attributed to microsomal monooxygenase activity induced by phenobarbitone and inhibited by piperonyl butoxide. 4-Methylpyrazole was found to inhibit both of these processes in vitro and when administered (90 mg kg−1 body weight) to rats, 2 hours prior to 1,3-difluoro-2-propanol, eliminated signs of poisoning, prevented (-) erythrofluorocitrate synthesis, and markedly decreased citrate and fluoride accumulation in vivo. 4-Methylpyrazole also appeared to diminish (-) erythrofluorocitrate synthesis from fluoroacetate in vivo, and this was attributed to its capacity to inhibit malate dehydrogenase activity. The antidotal potential of 4-methylpyrazole and the potential for 1,3-difluoro-2-propanol to replace fluoroacetate (compound 1080) as a vertebrate pesticide is discussed.

Item Type: Journal Article
Murdoch Affiliation: School of Biological Sciences and Biotechnology
Publisher: John Wiley and Sons Inc.
Copyright: © 1997 John Wiley & Sons, Inc.
URI: http://researchrepository.murdoch.edu.au/id/eprint/18111
Item Control Page Item Control Page