Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

cDNA generation on paramagnetic beads

Wang, Z. and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (2003) cDNA generation on paramagnetic beads. In: Generation of cDNA Libraries: Methods in Molecular Biology. Humana Press, Totowa, NJ, pp. 25-31.

Link to Published Version: http://dx.doi.org/10.1385/1-59259-359-3:25
*Subscription may be required

Abstract

Synthesis of complementary DNA (cDNA) by reverse transcription (RT) is a key step in investigating specific gene expression of a single transcript by RT-PCR (polymerase chain reaction) or to study the more complex profiles of gene expression in a biological sample using cDNA library or other techniques. Solid-phase generation of cDNA (1), based on immobilization of the synthesized cDNA on paramagnetic beads and the magnetic-bead separation technology, is a very useful method for studying gene expression particularly when handling limited amounts of starting materials (see Note 1). Using this method, analysis of gene expression can be carried out at a single-cell level (2–4). This is not possible using conventional techniques of mRNA isolation and cDNA cloning.

Item Type: Book Chapter
Murdoch Affiliation: Western Australian State Agricultural Biotechnology Centre
Publisher: Humana Press
Copyright: © Humana Press Inc.
URI: http://researchrepository.murdoch.edu.au/id/eprint/16813
Item Control Page Item Control Page