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cDNA generation on paramagnetic beads

Wang, Z. and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (2003) cDNA generation on paramagnetic beads. In: Generation of cDNA Libraries: Methods in Molecular Biology. Humana Press, Totowa, NJ, pp. 25-31.

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Synthesis of complementary DNA (cDNA) by reverse transcription (RT) is a key step in investigating specific gene expression of a single transcript by RT-PCR (polymerase chain reaction) or to study the more complex profiles of gene expression in a biological sample using cDNA library or other techniques. Solid-phase generation of cDNA (1), based on immobilization of the synthesized cDNA on paramagnetic beads and the magnetic-bead separation technology, is a very useful method for studying gene expression particularly when handling limited amounts of starting materials (see Note 1). Using this method, analysis of gene expression can be carried out at a single-cell level (2–4). This is not possible using conventional techniques of mRNA isolation and cDNA cloning.

Item Type: Book Chapter
Murdoch Affiliation: Western Australian State Agricultural Biotechnology Centre
Publisher: Humana Press
Copyright: © Humana Press Inc.
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