cDNA generation on paramagnetic beads
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Wang, Z. and Jones, M.G.K.
ORCID: 0000-0001-5002-0227
(2003)
cDNA generation on paramagnetic beads.
In:
Generation of cDNA Libraries: Methods in Molecular Biology.
Humana Press, Totowa, NJ, pp. 25-31.
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Abstract
Synthesis of complementary DNA (cDNA) by reverse transcription (RT) is a key step in investigating specific gene expression of a single transcript by RT-PCR (polymerase chain reaction) or to study the more complex profiles of gene expression in a biological sample using cDNA library or other techniques. Solid-phase generation of cDNA (1), based on immobilization of the synthesized cDNA on paramagnetic beads and the magnetic-bead separation technology, is a very useful method for studying gene expression particularly when handling limited amounts of starting materials (see Note 1). Using this method, analysis of gene expression can be carried out at a single-cell level (2–4). This is not possible using conventional techniques of mRNA isolation and cDNA cloning.
| Item Type: | Book Chapter |
|---|---|
| Murdoch Affiliation: | Western Australian State Agricultural Biotechnology Centre |
| Publisher: | Humana Press |
| Copyright: | © Humana Press Inc. |
| URI: | http://researchrepository.murdoch.edu.au/id/eprint/16813 |
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