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The effects of MCC-134 on the ATP-sensitive K+ channels in pig urethra

Yunoki, T., Teramoto, N., Takano, N., Seki, N., Creed, K.E., Naito, S. and Ito, Y. (2003) The effects of MCC-134 on the ATP-sensitive K+ channels in pig urethra. European Journal of Pharmacology, 482 (1-3). pp. 287-295.

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We have investigated the effects of MCC-134 (1-[4-(1H-imidazol-1-yl)benzoyl]-N-methyl-cyclobutanecarbothioamide) on membrane currents and ATP-sensitive K+ channel (KATP channel) opener-induced currents in pig urethra by use of patch-clamp techniques (conventional whole-cell configuration and nystatin perforated patch recordings). Tension measurement was also performed to study the effects of MCC-134 on the resting tone of pig urethral strips. MCC-134 reduced the resting tone of pig urethra in a concentration-dependent manner (EC50=6 μM). The MCC-134 (30 μM)-induced relaxation was suppressed by glibenclamide. In voltage-clamp experiments, MCC-134 produced a concentration-dependent inward K+ current which was suppressed by application of glibenclamide at a holding potential of −50 mV (symmetrical 140 mM K+ conditions). Application of MCC-134 enhanced diazoxide-induced inward currents and inhibited pinacidil-induced inward currents in a concentration-dependent manner at −50 mV. These results suggest that MCC-134 induces glibenclamide-sensitive KATP currents in pig urethra.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Publisher: Elsevier
Copyright: 2003 Elsevier BV
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