Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

The effects of MCC-134 on the ATP-sensitive K+ channels in pig urethra

Yunoki, T., Teramoto, N., Takano, N., Seki, N., Creed, K.E., Naito, S. and Ito, Y. (2003) The effects of MCC-134 on the ATP-sensitive K+ channels in pig urethra. European Journal of Pharmacology, 482 (1-3). pp. 287-295.

Link to Published Version: http://dx.doi.org/10.1016/j.ejphar.2003.09.068
*Subscription may be required

Abstract

We have investigated the effects of MCC-134 (1-[4-(1H-imidazol-1-yl)benzoyl]-N-methyl-cyclobutanecarbothioamide) on membrane currents and ATP-sensitive K+ channel (KATP channel) opener-induced currents in pig urethra by use of patch-clamp techniques (conventional whole-cell configuration and nystatin perforated patch recordings). Tension measurement was also performed to study the effects of MCC-134 on the resting tone of pig urethral strips. MCC-134 reduced the resting tone of pig urethra in a concentration-dependent manner (EC50=6 μM). The MCC-134 (30 μM)-induced relaxation was suppressed by glibenclamide. In voltage-clamp experiments, MCC-134 produced a concentration-dependent inward K+ current which was suppressed by application of glibenclamide at a holding potential of −50 mV (symmetrical 140 mM K+ conditions). Application of MCC-134 enhanced diazoxide-induced inward currents and inhibited pinacidil-induced inward currents in a concentration-dependent manner at −50 mV. These results suggest that MCC-134 induces glibenclamide-sensitive KATP currents in pig urethra.

Item Type: Journal Article
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Publisher: Elsevier
Copyright: 2003 Elsevier BV
URI: http://researchrepository.murdoch.edu.au/id/eprint/16570
Item Control Page Item Control Page