Tissue culture of Ecklonia radiata (Phaeophyceae, Laminariales): effects on growth of light, organic carbon source and vitamins

Lawlor, H.J., McComb, J.A. and Borowitzka, M.A.ORCID: 0000-0001-6504-4563 (1989) Tissue culture of Ecklonia radiata (Phaeophyceae, Laminariales): effects on growth of light, organic carbon source and vitamins. Journal of Applied Phycology, 1 (2). pp. 105-112.

Abstract

The effects of light quality and irradiance, and supply of organic carbon and vitamins on the growth of two forms of Ecklonia radiata in tissue culture were examined. A callus of unpigmented cells developed over the cut surface of newly excised explants of stipe. This growth was best in the dark but stopped after 10 weeks. Pigmented, mainly filamentous clumps of cells developed from explants after several weeks in culture. These required light for growth, with growth being enhanced by increasing photon flux density up to 30 μmol photon m-2 s-1, with the active spectral component being red light (> 600 nm). The addition to the medium of a range of organic carbon sources or vitamins did not stimulate growth of either culture type in the dark.

Item Type:	Journal Article
Murdoch Affiliation(s):	School of Biological and Environmental Sciences
Publisher:	Springer Verlag
Copyright:	© 1989 Kluwer Academic Publishers
URI:	http://researchrepository.murdoch.edu.au/id/eprint/16498

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