Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.)

Bian, M., Waters, I., Broughton, S., Zhang, X-Q, Zhou, M., Lance, R., Sun, D. and Li, C. (2013) Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.). Molecular Breeding, 32 (1). pp. 155-164.

Link to Published Version:
*Subscription may be required


Acid soil/aluminium toxicity is one of the major constraints on barley production around the world. Genetic improvement is the best solution and molecular-marker-assisted selection has proved to be an efficient tool for developing barley cultivars with acid soil/aluminium tolerance. In this study, barley variety Svanhals-introduced from CYMMIT (International Maize and Wheat Improvement Center)-was identified as acid soil/aluminium tolerant and the tolerance was mapped to chromosome 4H in 119 doubled haploid (DH) lines from a cross of Hamelin/Svanhals. The HvMATE gene, encoding an aluminium-activated citrate transporter, was selected as a candidate gene and gene-specific molecular markers were developed to detect acid soil/aluminium tolerance based on the polymerase chain reaction. Sequence analysis of the HvMATE gene identified a 21-bp indel (insertion-deletion) between the tolerant and sensitive cultivars. The new marker was further mapped to the QTL (quantitative trait loci) region on chromosome 4H for acid soil tolerance and accounted for 66.9 % of phenotypic variation in the DH population. Furthermore, the polymorphism was confirmed in other tolerant varieties which have been widely used as a source of acid soil tolerance in Australian barley breeding programs. The new gene-specific molecular marker provides an effective and simple molecular tool for selecting the acid soil tolerance gene from multiple tolerance sources.

Item Type: Journal Article
Murdoch Affiliation(s): Western Australian State Agricultural Biotechnology Centre
Publisher: Kluwer Academic Publishers
Copyright: © 2013 Springer Science+Business Media Dordrecht
Item Control Page Item Control Page