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Application of immunomagnetic bead technology for improved diagnosis of classical swine fever virus

Conlan, J., Khounsy, S., Phruaravanh, M., Soukvilai, V., Morrissy, C., Colling, A., Blacksell, S., Wilks, C. and Gleeson, L. (2008) Application of immunomagnetic bead technology for improved diagnosis of classical swine fever virus. In: Conlan, J., Blacksell, S., Morrissy, C. and Colling, A., (eds.) Management of classical swine fever and foot-and-mouth disease in Lao PDR : proceedings of an international workshop held in Vientiane, Lao PDR, 20-21 November 2006. ACIAR Proceedings No. 128. ACIAR, Canberra, Australia, pp. 74-79.

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Classical swine fever (CSF) is a highly contagious viral disease of swine that causes major losses in all pig production systems in many regions of the world. In Lao PDR, CSF is endemic and outbreaks have adverse effects on the predominantly smallholder farming sector. Laboratory testing is required to accurately identify CSF outbreaks because of the difficulty of making a diagnosis based solely on clinical signs. The National Animal Health Centre, located in the national capital, Vientiane, has the capacity to reliably detect CSF antigen in tissue samples using an antigen capture (AC)-ELISA, and antibodies to CSF virus from serum samples using the complex trapping blocking ELISA. This paper describes the use of immunomagnetic beads (IMB) as the solid phase for the portable detection of CSF antigen in spleen samples and for the reliable detection of antibodies to CSF in animals vaccinated with a lapinised C-strain vaccine. The portable IMB-ELISA for antigen detection was shown to be 100% sensitive and 91% specific in comparison to the AC-ELISA. The IMB-Antibody-ELISA was shown to be 97% sensitive and 95% specific in comparison to the gold standard—neutralising peroxidase linked assay. These new diagnostic tests have the potential to improve CSF management through portable and rapid identification of outbreaks and the reliable and inexpensive monitoring of vaccination programs.

Item Type: Book Chapter
Publisher: ACIAR
Copyright: © Commonwealth of Australia 2008
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