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Burkholderia rhynchosiae sp. nov. isolated from Rhynchosia ferulifolia root nodules

De Meyer, S.E., Cnockaert, M., Ardley, J.K., Trengove, R.D., Garau, G., Howieson, J.G. and Vandamme, P. (2013) Burkholderia rhynchosiae sp. nov. isolated from Rhynchosia ferulifolia root nodules. International Journal of Systematic and Evolutionary Microbiology, 63 (11). pp. 3944-3949.

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Two strains of Gram-stain-negative rod-shaped bacteria were isolated from root nodules of the South African legume Rhynchosia ferulifolia and authenticated on this host. Based on phylogenetic analysis of the 16S rRNA gene, strains WSM3930 and WSM3937T belonged to the genus Burkholderia, with the highest degree of sequence similarity to Burkholderia terricola (98.84%). Additionally, the housekeeping genes gyrB and recA were analysed since 16S rRNA gene sequences are highly similar between closely related Burkholderia species. The results obtained for both housekeeping genes showed the highest degree of sequence similarity towards Burkholderia caledonica LMG 19077T (94.2% and 94.5%, respectively). Chemotaxonomic data, including fatty acid profiles and respiratory quinone data supported the assignment of strains WSM3930 and WSM3937T to the genus Burkholderia. DNA-DNA hybridisations, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains WSM3930 and WSM3937T from the most closely related validly named Burkholderia species. We conclude therefore that these strains represent a novel species for which the name Burkholderia rhynchosiae sp. nov. is proposed, with strain WSM3937T = LMG 27174T = HAMBI 3354T as type strain.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Rhizobium Studies
Publisher: Society for General Microbiology
Copyright: © 2013 IUMS
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