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Burkholderia sprentiae sp. nov. isolated from Lebeckia ambigua root nodules

De Meyer, S.E., Cnockaert, M., Ardley, J.K., Maker, G.ORCID: 0000-0003-1666-9377, Yates, R., Howieson, J.G. and Vandamme, P. (2013) Burkholderia sprentiae sp. nov. isolated from Lebeckia ambigua root nodules. International Journal of Systematic and Evolutionary Microbiology, 63 (11). pp. 3950-3957.

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Seven gram-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM5005T being most closely related to Burkholderia tuberum (98.08% sequence similarity). Additionally, these strains formed a distinct group in phylogenetic trees based on the housekeeping genes gyrB and recA. Chemotaxonomic data including fatty acid profiles and analysis of respiratory quinones supported the assignment of the strains to the genus Burkholderia. Results of DNA-DNA hybridisations, physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from the closest validly named Burkholderia neighbour species. Therefore, these strains represent a novel species for which the name Burkholderia sprentiae sp. nov. (WSM5005T = LMG 27175T = HAMBI 3357 T), is proposed.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Rhizobium Studies
Publisher: Society for General Microbiology
Copyright: © 2013 IUMS
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