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Use of a whole chromosomal probe for identification of Treponema hyodysenteriae

Combs, B.G. and Hampson, D.J.ORCID: 0000-0002-7729-0427 (1991) Use of a whole chromosomal probe for identification of Treponema hyodysenteriae. Research in Veterinary Science, 50 (3). pp. 286-289.

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A whole chromosomal DNA probe labelled with photobiotin was used in a dot blot hybridisation to identify DNA from isolates of Treponema hyodysenteriae, the aetiological agent of swine dysentery. The probe was evaluated using DNA from 13 isolates of T hyodysenteriae and 13 isolates of non-T hyodysenteriae spirochaetes recovered from pigs. The initial test had both a sensitivity and specificity of 92·3 per cent, although when it was repeated the specificity fell to 84·6 per cent. The test was helpful in distinguishing between T hyodysenteriae and other morphologically similar treponemes that are part of the normal flora in the large intestine of pigs. The probe could also be used to detect as little as 10 ng of purified DNA from T hyodysenteriae, or DNA from 2 × 106 bacterial cells lysed directly onto nitrocellulose.

Item Type: Journal Article
Murdoch Affiliation(s): School of Veterinary Studies
Publisher: Harcourt Publishers Ltd
Copyright: © 1991 Elsevier Ltd.
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