Localization of methyl-coenzyme M reductase as metabolic marker for diverse methanogenic archaea

Wrede, C., Walbaum, U., Ducki, A., Heieren, I. and Hoppert, M. (2013) Localization of methyl-coenzyme M reductase as metabolic marker for diverse methanogenic archaea. Archaea, 2013 .

Abstract

Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms. Here, the potential of MCR-specific polyclonal antibodies as metabolic marker in various methanogenic Archaea is shown. For standard growth conditions in laboratory culture, the cytoplasmic localization of the enzyme in Methanothermobacter marburgensis, Methanothermobacter wolfei, Methanococcus maripaludis, Methanosarcina mazei, and in anaerobically methane-oxidizing biofilms is demonstrated. Under growth limiting conditions on nickel-depleted media, at low linear growth of cultures, a fraction of 50-70% of the enzyme was localized close to the cytoplasmic membrane, which implies "facultative" membrane association of the enzyme. This feature may be also useful for assessment of growth-limiting conditions in microbial biofilms.

Item Type:	Journal Article
Murdoch Affiliation(s):	School of Veterinary and Life Sciences
Copyright:	Hindawi Publishing Corporation
Notes:	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI:	http://researchrepository.murdoch.edu.au/id/eprint/14347

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