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Strongyloides ratti: Mitochondrial enzyme activities of the classical electron transport pathway in the infective (L3) larvae

Armson, A., Grubb, W.B. and Mendis, A.H.W. (1995) Strongyloides ratti: Mitochondrial enzyme activities of the classical electron transport pathway in the infective (L3) larvae. International Journal for Parasitology, 25 (2). pp. 257-260.

Link to Published Version: http://dx.doi.org/10.1016/0020-7519(94)E0062-R
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Abstract

Submitochondrial particles prepared from S. ratti L3 larvae exhibited NADH-oxidase (NOX), NADH-ferricyanide reductase (NFR), NADH-cytochrome-c-reductase (NCR), succinate-cytochrome-c-reductase (SCR), and cytochrome-aa3-oxidase activities of 2.1 ± 0.3, 8.9 ± 1.3, 0.6 ± 0.1, 1.0 ± 0.2 and 1.2 ± 0.3 nm min−1 mg protein−1 respectively, at 37°C. The NCR and NOX activities were 39.3% and 23.5% of the NFR activity, suggesting the occurrence of a rate-limiting step or bifurcation of the respiratory electron transport (RET) pathway on the oxygen-side of RET-Complex I. The NCR activity was 50% that of cytochrome-aa3-oxidase activity which suggests partitioning of electron flow at the level of RET-Complex III and/or the quinone-function. Antimycin A and rotesone but not 2-thenoyl trifluoreacetone (TTFA) inhibited NCR activity, the EC50 values were 3.6 × 10−6m, 3.7 × 10−7m, respectively. SCR activity was inhibited by antimycin A (EC50 = 3.8 × 10−6m) and TTFA (EC50 = 2.8 × 10−5m) but not by rotenone. The results suggest that presence of classical and alternate RET-pathways in S. ratti L3 larvae.

Item Type: Journal Article
Publisher: Elsevier BV
Copyright: 1995 Australian Society for Parasitology
URI: http://researchrepository.murdoch.edu.au/id/eprint/14228
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