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Reverse vaccinology approach to identify Rhipicephalus (Boophilus) microplus tick vaccine candidates

Lew-Tabor, A.E., Jackson, L.A., Rodriguez-Valle, M., Kurscheid, S., Moolhuijzen, P.M., Jones, M.E., Piper, E.K., Constantinoiu, C., Jorgensen, W.K., Jonsson, N.N., Bellgard, M.I. and Guerrero, F.D. (2008) Reverse vaccinology approach to identify Rhipicephalus (Boophilus) microplus tick vaccine candidates. In: ASP & ARC/NHMRC Research Network for Parasitology Annual Conference, 6 - 9 July 2008, Glenelg, S.A.


Of 899 tick species [subclass Acari, order Parasitiformes], approximately 50 are significant medical and veterinary ectoparasites. Rhipicephalus microplus [cattle tick, family Ixodidae] infestations impacts cattle production costing $US2.5bn world-wide. Capitalising on an EST database (BmiGI), novel ESTs from suppressive subtractive hybridizations, Ixodes scapularis genome and bioinformatics, we are applying a reverse vaccinology approach and have identified 208 putative tick vaccine candidates. Predicted proteins include lipocalins, proteases, protease inhibitors, lipoprotein receptors, extracellular matrix, membrane, cuticle enzymes, chitin binding and 146 proteins of unknown function. In parallel, we have undertaken an extensive immune and gene expression analysis of cattle with differential responses to ticks, and have developed novel in vitro candidate screening tools. Epitope screening, functional analysis and the ability to direct protective immune responses will further refine the candidates for in vivo screening.

Item Type: Conference Paper
Murdoch Affiliation(s): Centre for Comparative Genomics
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