Albumin and globulin dynamics during grain development of elite Chinese wheat cultivar Xiaoyan 6

Dong, K., Ge, P., Ma, C., Wang, K., Yan, X., Gao, L., Li, X., Liu, J., Ma, W.ORCID: 0000-0002-1264-866X and Yan, Y. (2012) Albumin and globulin dynamics during grain development of elite Chinese wheat cultivar Xiaoyan 6. Journal of Cereal Science, 56 (3). pp. 615-622.

Abstract

Proteome Dynamics of albumins and globulins during grain development of elite Chinese wheat cultivar Xiaoyan 6 were studied. A total of 210 differentially expressed protein spots were recognized by two-dimensional differential gel electrophoresis (2D-DIGE) and 146 of these that represented 89 unique proteins were identified by MALDI-TOF mass spectrometry. Four protein expression patterns were observed across five grain developmental stages. Among the identified proteins, more than 80% were enzymes in eight functional categories, including carbohydrate metabolism (27%), protein metabolism (27%), stress/defense/detoxification (11%), cell metabolism (6%), transcription/translation (4%), nitrogen metabolism (4%), photosynthesis (4%), and signal transduction (1%). Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 18 representative genes demonstrated that about two thirds of the genes displayed different expression profiles between the transcriptional and translational stages.

Item Type:	Journal Article
Murdoch Affiliation(s):	Western Australian State Agricultural Biotechnology Centre
Publisher:	Academic Press
Copyright:	© 2012 Elsevier Ltd.
URI:	http://researchrepository.murdoch.edu.au/id/eprint/11600

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