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Evaluation of the dual IFNγ/IL-2 fluorospot-assay with flow cytometry for detection of HLA-restricted HIV-specific T-cell responses in HIV controllers

Keane, N., Almeida, C., Roberts, S., Ahmad, I., Mallal, S. and John, M. (2012) Evaluation of the dual IFNγ/IL-2 fluorospot-assay with flow cytometry for detection of HLA-restricted HIV-specific T-cell responses in HIV controllers. Retrovirology, 9 (Suppl 2). P243.

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Link to Published Version: http://dx.doi.org/10.1186/1742-4690-9-S2-P243
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Abstract

The IFNγ ELISpot assay is used widely for high through-put screening of HIV-specific responses in studies of HIV infection and vaccine studies. However, dual production of IFNγ/IL-2 and increased proliferative capacity may be associated with better natural control of HIV infection. We evaluated a novel fluorospot assay enabling the identification of dual IFNγ/IL-2 producing antigen-specific cells and compared it with intracellular cytokine staining by standard flow cytometry in individuals with natural control of HIV-infection.

Item Type: Journal Article
Murdoch Affiliation(s): Institute for Immunology and Infectious Diseases
Publisher: BioMed Central
Copyright: © 2012 Keane et al
Notes: Poster Presentation at AIDS Vaccine 2012, Boston, 9-12 September, 2012.
URI: http://researchrepository.murdoch.edu.au/id/eprint/10928
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