Quantitative disease resistance assessment by real-time PCR using the Stagonospora nodorum-wheat pathosystem as a model
Oliver, R.P., Rybak, K., Shankar, M., Loughman, R., Harry, N. and Solomon, P.S. (2008) Quantitative disease resistance assessment by real-time PCR using the Stagonospora nodorum-wheat pathosystem as a model. Plant Pathology, 57 (3). pp. 527-532.
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Measuring disease resistance accurately and reproducibly is a key requirement for the introgression of partial resistance genes into breeding lines. Here, a qPCR protocol is used to measure fungal biomass, using the wheat-Stagonospora nodorum pathosystem as a model. Seven cultivars of differing reported resistance levels were used. Fungal biomass taken at 220°C thermal days after inoculation accurately predicts the final grain weight loss. It is concluded that a test based on qPCR methods is specific, quantitative, rapid and objective. Such tests could provide useful and economic tools in the development of robustly resistant crop cultivars.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||Australian Centre for Necrotrophic Fungal Pathogens|
|Copyright:||2008 The Authors|
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